Fluorescent dUTP helps characterize 10 novel tetranucleotide microsatellites from an enriched salamander
نویسنده
چکیده
Ten tetranucleotide microsatellite loci were isolated and characterized from Indiana (USA) populations of the smallmouth salamander, Ambystoma texanum . As opposed to individually labelling primers that were not yet known to be polymorphic, we used fluorescent dUTP to assess genetic variability and found it to be very effective. Allelic diversity ranged from two to 18 alleles per locus and observed heterozygosity ranged from 0.17 to 0.91 among roughly 25 individuals. One of the 10 markers also amplified and was polymorphic in the tiger salamander ( A. tigrinum ).
منابع مشابه
Polymorphic tetranucleotide microsatellites for Cope's giant salamander (Dicamptodon copei) and Pacific giant salamander (Dicamptodon tenebrosus).
We present primers and amplification conditions for 15 microsatellite loci developed for the Cope's giant salamander (Dicamptodon copei), 14 of which are tetranucleotide repeats. Cross-species amplification revealed 10 of these loci to also be polymorphic in the Pacific giant salamander (Dicamptodon tenebrosus). Several loci produced nonoverlapping allelic ranges between the two species and may...
متن کاملDistribution of Polymorphic and Non-Polymorphic Microsatellite Repeats in Xenopus tropicalis
The results of our bioinformatics analysis have found over 91,000 di-, tri-, and tetranucleotide microsatellites in our survey of 25% of the X. tropicalis genome, suggesting there may be over 360,000 within the entire genome. Within the X. tropicalis genome, dinucleotide (78.7%) microsatellites vastly out numbered tri- and tetranucleotide microsatellites. Similarly, AT-rich repeats are overwhel...
متن کاملIsolation and characterization of microsatellites in lane
Polymerase chain reaction primer pairs for a total of 25 nuclear-encoded microsatellites (loci) were developed from genomic DNA libraries of lane snapper ( Lutjanus synagris ), mutton snapper ( Lutjanus analis ), and yellowtail snapper ( Ocyurus chrysurus ). The microsatellites include 24 perfect (21 dinucleotide and three trinucleotide) and one imperfect (combination tetranucleotide/tetranucle...
متن کاملCharacterization of Tri- and Tetranucleotide Microsatellites in the Black Tiger Prawn, Penaeus monodon
In order to characterize triand tetranucleotide microsatellites in the black tiger prawn, Penaeus monodon, a genomic library of P. monodon was screened with the repeats (GAA)n, (GATA)n, (GGAT)n, (GGAA)n and (CACC)n. In the 79 positive clones isolated, the repeats (GATA)n was found at the highest frequency, followed by the (GAA)n while the other sequence types were rare or not found. Sequencing ...
متن کاملIsolation and characterization of microsatellite markers in the Serra Spanish mackerel, Scomberomorus brasiliensis.
Thirteen nuclear-encoded microsatellites from a genomic DNA library of Serra Spanish mackerel, Scomberomorus brasiliensis, were isolated and characterized. The microsatellites include 10 perfect repeats (eight tetranucleotide and two dinucleotide) and three imperfect repeats (two tetranucleotide and one dinucleotide). An additional five microsatellites, isolated originally from two congeneric s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2004